OE24

Heute ist der 23.05.2025

Datum: 23.05.2025 - Source 1 (https://www.oe24.at/welt/sensations-studie-erstmals-aura-eines-lebewesens-von-forschern-fotografiert/634021016):
- Forschungsteam aus Kanada hat ein schwaches Leuchten um lebende Organismen sichtbar gemacht, das erlischt, wenn sie sterben.
- Erkenntnisse könnten helfen, biologische Prozesse besser zu verstehen und zu untersuchen.
- Auch Pflanzen und Pilze zeigen dieses Verhalten.
- Studie veröffentlicht im Fachmagazin Journal of Physical Chemistry Letters.
- Verwendung von EMCCD-Kameras, die extrem schwache Lichtsignale erfassen können.
- Messungen fanden in abgedunkelten Räumen statt, um äußere Störungen zu vermeiden.
- Tests mit Mäusen unter Vollnarkose: schwaches Leuchten nachweisbar, solange die Tiere lebten; erlischt nach dem Tod.
- Ähnliche Tests mit der Pflanze Ackerschmalwand: Leuchten verstärkt sich bei Stress (Alkohol, Wasserstoffperoxid, hohe Temperaturen).
- Pilze zeigten ähnliche Reaktionen wie Pflanzen.
- Licht entsteht vermutlich durch molekulare Reaktionen in Zellen, bei denen Stoffe zerfallen und kleine Lichtblitze (Biophotonen) entstehen.
- Biophotonen treten besonders bei Stress oder Schmerzen auf.
- Erste fotografische Darstellung der Lichtverteilung auf der Oberfläche lebender Organismen.
- Mögliche zukünftige Anwendungen: Beobachtung der Vitalität von Lebewesen in Landwirtschaft und biologischer Forschung.

Source 2 (https://andor.oxinst.com/learning/view/article/4-5d-microscopy):
- Rapid, multi-dimensional imaging of living cells or model organisms is increasingly necessary in biological research.
- Fundamental biological phenomena involve microscopic structures moving rapidly in 3D space.
- Effective real-time capture of these processes requires 4D imaging (3D spatial dimensions + time).
- To track multiple intracellular structures, 5D imaging (multiple spectrally separated fluorophores) is often needed.
- Key goals in imaging include minimizing phototoxic damage to cells/tissues and photobleaching of fluorophores.
- Techniques for 5D cell imaging include:
- Spinning disk confocal microscopy
- Deconvolution widefield microscopy
- Structured illumination
- Electron Multiplying CCD (EMCCD) technology, such as the iXon EMCCD Camera, enhances these imaging techniques.
- EMCCD technology offers a high signal-to-noise ratio compared to conventional detectors.
- Rapid-readout capabilities of EMCCD allow for quick acquisition of z-stacks and time-lapse sequences.
- Spectral dimension can be accessed through fast switching between fluorophores or simultaneous emission signal splitting.
- Minimizing excitation powers reduces dye photobleaching and cell phototoxicity.
- iXon3 EMCCD Camera and Luca EMCCD platforms provide single photon sensitivity and high Quantum Efficiency at multi-MHz readout speeds.

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